Device

Part:BBa_K283020

Designed by: W Ling , Nelson Chu, Shi Lei   Group: iGEM09_HKU-HKBU   (2009-10-13)

FF_FF ansB promoter-rbs-cheZ_histag-terminators

This part consist of a CheZ protein linked to a double terminator. The CheZ protein is responsible for the dephosphorylation of CheY protein in bacteria flagella movement. The CheZ coding region has a 6-Histidine tail and do not contain any rbs.This part does not pose any biological threat.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 777
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 28
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme/phosphorylation
//function/motility
Parameters
None